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. 2016 Oct 21;7(48):78713–78725. doi: 10.18632/oncotarget.12805

Figure 3. SIN3 paralogs inversely regulate invasion and metastasis.

Figure 3

(A) MDA-MB-231 cells were seeded into the inserts of 24-well plates coated with Matrigel in serum-free media. Photomicrographs were taken after 22-hour incubation period and staining with 1% crystal violet. The same shRNA constructs shown in Figure 2E were utilized. Scale bar is 50 μm. (B) The number of invaded cells was quantified. (C) MDA-MB-231 cells were injected into athymic mice via lateral tail vein. Lungs were collected 8 weeks post injection and stained in Bouin's fixative. Metastases per lung were counted and the average number was normalized to the parent control. Number of mice (n) is listed above each group. *indicates p < 0.05 compared to control.