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. Author manuscript; available in PMC: 2017 Mar 7.
Published in final edited form as: Cancer Stud Mol Med. 2015 Dec 9;2(2):87–95. doi: 10.17140/CSMMOJ-2-113

Figure 1.

Figure 1

Cell density affects ALDEFLUOR activity without changing other stem cell characteristics. Representative images of HT29 and SW480 cells grown ay low (30–40%) and high (70–80%) cell densities (A). It is important to note that the cells never reach full confluence before analysis. Cells from low and high density were cultured and ALDEFLUOR assay was performed. Representative histograms from one set of analysis on HT29 and SW480 cells grown at low and high density (B). Different colon cancer cell lines were cultured at low and high densities and ALDEFLUOR was performed. The average values of percent positive cells is graphed to show significant changes in ALDEFLUOR positive cells at low and high densities (C). Expression of CD166 on three different colon cancer cell lines grown at low and high densities (D). Cells were grown at low and high density and then plated for colonosphere assay. Graph represents average number of spheres formed under each culture condition (E). All experiments were N=3 except ALDEFLUOR assay was performed with multiple relicates (N=10). *=p<0.05, **= p<0.01, ***=p<0.001.