|
| |
| Components | Volume |
|
| |
| 5× Go-Taq PCR buffer | 10 μL |
|
| |
| Taq polymerase (5 units/μL) | Taq polymerase (5 units/μL) |
|
| |
| Genomic DNA | 2 μL [100 ng (50 ng/μL)] |
|
| |
| For-pSM2 (10 pmoles/μL) | 1 μL |
|
| |
| Rev-pSM2 (10 pmoles/μL) | 1 μL |
|
| |
| dd H2O | 35 μL |
|
| |
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