Table 1.
Sequences 5′ to 3′ | |
---|---|
Primers with Illumina adapters: | |
Adap 1 L 1HsG | CAAGCAGAAGACGGCATAOGAGCTCTTCCGATC TTGCACATGAOCCTAAAACTTAG |
Adap2Seq1 | AATGATACGGCGACCACCGAGATCTACACTTTOCC TACACGACGACGCTCTTCCGATCT |
L1 specific primers: | |
L1HsSP1A2 | GGGAGATATACCTAATGCTAGATGACAC (specific for L 1Hs subset) |
L1 “G” primer | TGCACATGTACOCTAAAACTTAG (specific for L 1Hs subset) |
L1nt112out | GATGAACCCGTACCTCAGA |
Degenerate primers: | |
DEG Seq 1N5TCTGT | ACACTCTTTCCCTACACGACGACGCTCTTCCGA TCTNNNNNTCTGT |
DEGSeq1N5CTTCT | ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN NNNCTTCT |
DEGSeq1N5TGCCT | ACACTCTTTCCCTACACGACGACGCTCTTCCGAT CTNNNNNTGCCT |
DEGSeq1NTCTCA | ACACTCTTTCCCTACACGACGCTCTTCCGATCTN NNNNTCTCA |
DEGSeq1N5CAGAG | ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN NNNCAGAG |
DEGSeq1N5TTGAA | ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN NNNTTGAA |
DEGSeq1N5CTTTG | ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN NNNCTTTG |