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. Author manuscript; available in PMC: 2017 Mar 7.
Published in final edited form as: Methods Mol Biol. 2016;1400:79–93. doi: 10.1007/978-1-4939-3372-3_5

Table 1.

Primers used for L1-seq

Sequences 5′ to 3′
Primers with Illumina adapters:
  Adap 1 L 1HsG CAAGCAGAAGACGGCATAOGAGCTCTTCCGATC
  TTGCACATGAOCCTAAAACTTAG
  Adap2Seq1 AATGATACGGCGACCACCGAGATCTACACTTTOCC
  TACACGACGACGCTCTTCCGATCT
L1 specific primers:
  L1HsSP1A2 GGGAGATATACCTAATGCTAGATGACAC (specific
  for L 1Hs subset)
  L1 “G” primer TGCACATGTACOCTAAAACTTAG (specific for L 1Hs
  subset)
  L1nt112out GATGAACCCGTACCTCAGA
Degenerate primers:
  DEG Seq 1N5TCTGT ACACTCTTTCCCTACACGACGACGCTCTTCCGA
  TCTNNNNNTCTGT
  DEGSeq1N5CTTCT ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN
  NNNCTTCT
  DEGSeq1N5TGCCT ACACTCTTTCCCTACACGACGACGCTCTTCCGAT
  CTNNNNNTGCCT
  DEGSeq1NTCTCA ACACTCTTTCCCTACACGACGCTCTTCCGATCTN
  NNNNTCTCA
  DEGSeq1N5CAGAG ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN
  NNNCAGAG
  DEGSeq1N5TTGAA ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN
  NNNTTGAA
  DEGSeq1N5CTTTG ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN
  NNNCTTTG