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. Author manuscript; available in PMC: 2017 Mar 7.
Published in final edited form as: Nat Cell Biol. 2016 Oct 24;18(11):1233–1243. doi: 10.1038/ncb3427

Figure 1.

Figure 1

Statins reduce levels of conformational or misfolded mutp53. (a) Western blotting (WB) for p53 and β-actin using SK-Br-3 (p53R175H) and U2OS (p53wt) cells treated with the indicated concentrations of DMSO (D) or lovastatin (L) for 24 h. (b) WB for p53 and β-actin or vinculin using SK-Br-3 and U2OS cells treated with the indicated concentrations of DMSO (D) or atorvastatin (A) for 24 h. (c) WB for the indicated proteins using KHOS/NP (p53R156P), BxPC-3 (p53Y220C), HT29 (p53R273H) and MDA-MB-231 (p53R280K) cells treated with D or L for 24 h. (d) Immunoprecipitation (IP) for total p53 (DO1), the folded/native form of p53R248W (PAb1620), and the misfolded/denatured form of p53R248W (PAb240), using MIA PaCa-2 cells treated with D or L, followed by WB for p53 (7F5). Additional results are shown in Supplementary Fig. 1. Unprocessed original scans of blots are shown in Supplementary Fig. 8.