Figure 2.

C-terminally truncated Gal4-tagged human ORF2p can drive variable levels of Alu retrotransposition. (A) Schematic of C-terminally truncated ORF2 constructs. Gal4 tag position indicated by blue-gray rectangle. (B) Western blot analysis of ORF2 fragments transiently transfected in HeLa cells. ORF2p fragments were detected using anti-Gal4 antibodies. Protein product of expected size is denoted by a * for each construct. Molecular weight markers are denoted at the left of the image. GAPDH was used as loading control. (C) Alu retrotransposition assay results. Neo^r colonies correspond to de novo retrotransposition events. Error bars denote SD of n = 3 experiments. Representative flasks for each ORF2 construct are shown above corresponding graph bars.