Figure 5.
rts1Δ mutation suppresses the anaphase entry delay in dam1-3D cells without compromising SAC activation. (A) The delay of Pds1 degradation in dam1-3D cells is suppressed by rts1Δ. G1-arrested WT and mutant cells with Pds1-18myc were released into YPD medium at 30°. α-factor was added back after budding. Cell lysates were prepared at the indicated time points for Western blotting with anti-myc antibody. The Pds1 levels and budding index are shown. Pgk1, loading control. (B) rts1∆ cells show intact SAC function. G1-arrested PDS1-18myc and rts1Δ PDS1-18myc cells were released into YPD medium containing 20 μg/ml of nocodazole and incubated at 30°. Cells were collected every 20 min for the budding index and the determination of Pds1 protein levels. Pgk1, loading control. The Pds1 levels and the budding index are shown.