Figure 4.

Transcript levels of Myc and related genes were assessed by qRT-PCR in feeding third instar larvae. (A) Myc transcript levels in whole larvae, as well as in isolated fat body tissue, are 1.7 times higher in female than in sibling male larvae. No significant differences in cycD or CanA14-F/Pp2B-14D transcript levels were observed between the sexes. (B) Transcripts of neither Mnt nor Max, the two other components of the Myc-Mnt-Max interactome, amass in a sexually dimorphic manner. Transcripts for the ribosomal proteins Rpl1 and Rpl135, known targets of Myc, are expressed equally in males and females as well. (C) Overall levels of Desat1 RNA are the same in both sexes but transcript levels are 1.5 times higher in male fat-body tissue compared to that of sibling females. (D) Male and female larvae with Cg-Gal4-driven expression of Desat1 in fat-body tissue are not significantly different in size than cocultured w¹¹¹⁸ control animals. * P < 0.02, *** P < 0.001; P-values were calculated using Student’s t-test. Error bars represent the standard deviation. n.s., not significant.