Fig 4. Cryopreserevd kidneys retain their laminin content even after decellularization.

Laminin immunohistostaining was carried out using anti laminin sera followed by FITC conjugated secondary IgG representing a) control kidney from group I, b) decellularized structures from freshly isolated kidney, group II and c) decellularized structure from cryostored kidney, group III at 200X. Where ever shown, the white arrows represented renal cells (blue stain) with black arrows representing laminin (brown stain). The cryostorage for 3 month minimally affected the histological appearance and overall presence of ECM component in the decellularized structure, group III in comparison to freshly de-celularized structure, group II.