Fig 5. Cellular level of Glycosaminoglaycans (GAGs) underwent a loss following decellularization and not the GAGs present in Extracellular matrix.

Alcian blue staining was used to localize the GAGs as shown in photomicrographs a) control kidney from group I, b) decellularized structures from freshly isolated kidney, group II and c) decellularized structure from cryostored kidney, group III at 200X. Where ever shown, the white arrows represented renal cells (black stain) and black arrows represented GAG staining (blue stain). A low content of staining was observed in the decellularized structures from group II and III in comparison to wild type kidneys, group I.