Fig 10. Decellularized scaffold were found to be nontoxic to murine C₃H₁₀T½ cells.

The MTT assay was performed to check if the SDS based decellularization of the kidneys bioscaffolds remained nontoxic to mesenchymal stem cells. The assay was performed under different conditions i.e. using only cells and media (i.e. without scaffold) as a positive control, cells with SDS in media as negative control, cells incubated with freshly isolated decellularized kidnyes (group II), and decellularized structures from cryostored kidnyes (group III). As shown in the graph, the SDS inhibited the growth of cells and for negative control the cell viability was only 9.29% while its was found to be 89.2% for the group II) and approximately 80% for the group III). Absorbance was measured in replicates of six and the calculated standard error of the mean (SEM) plotted error bars. Data are expressed as mean ±SEM. Based on ANOVA, significant differences among groups II, III w.r.t. control group I are indicated at *p<0.05.