Fig 1.
A) Representative confocal image stacks showing axons from WT, presynaptic overexpression of Par-1 (Par-1OE) and inactive Par-1 (Par-1T408A) using BG380-Gal4. Third instar larvae were stained with antibodies against BRP (Green), DVGLUT (Red) and HRP (Blue). Scale bar = 10μm B) Quantification of BRP intensity from axons in A. n = 12, **** = p<0.0001. C) Quantification of DVGLUT intensity from axons in A. n = 12, p = 0.12. D) Representative Western blots and bar graphs showing quantification of BRP levels in WT and Par-1OE brains. Syntaxin was used as a loading control. N = 3 p = 0.7124. E) Representative images and bar graphs showing quantification of VNCs stained with BRP (Red) from identical genotypes as in 1A. N = 12 p = 0.46. Scale bar = 10μm. Error bars represent S.E.M.