Fig. 3.

Retroviral transduction of Cxcr5 on Treg cells does not affect Treg cell signature genes expression or their suppressive ability. (A) Cell sorting strategy and sorting purity of retrovirally transduced Treg cells. After five days of retroviral transduction, CD4⁺RFP⁺GFP⁺ cells were sorted by flow cytometry. (B) Quantitative RT-PCR analysis of indicated genes were conducted in RV-empty vector- or RV-Cxcr5-transduced Treg cells. Naïve CD4⁺ T cells were included as a control. (C) Cell proliferation dye-labeled responder conventional T cells (Tconv; CD4⁺CD25^– T cells) were stimulated with anti-CD3 Ab in the presence of irradiated T cell-depleted splenocytes for 3 days. Titrated number of RV-empty vector- or RV-Cxcr5-transduced Treg cells were added to the culture. Cell proliferation was measured by flow cytometry. Data are representatives of three independent experiments (^**p<0.01).