Fig. 4.

CXCR5 expression in Cxcr5-transduced Treg cells is stable in vivo. (A) RV-empty vector- or RV-Cxcr5-transduced Treg cells were co-transferred with naïve CD4⁺ T cells isolated from B6.SJL congenic mice into Tcrb^−/− mice followed by subcutaneous immunization of the recipient mice with KLH. Seven days later, the transferred Treg cells in the draining lymph nodes were analyzed by flow cytometry after gating on CD4⁺CD45.2⁺GFP⁺ cells. (B, C) RFP expression in gated CD4⁺CD45.2⁺RFP⁺ were analyzed. (D, E) Flow cytometric analysis of CXCR5 expression in gated CD4⁺CD45.2⁺RFP⁺ cells. Data are representatives of two independent experiments (^**p<0.01).