Fig. 3.

Auranofin induces apoptosis in PC-3 cells. (A) Cell viability assay. PC-3 cells were treated with auranofin at various concentrations (0, 0.25, 0.5, or 1 μM) for 24 h. After incubation, the absorbance was measured at 450 nm. The percentage of cells surviving in each group relative to the control was calculated. The data are showed as a mean ± SD (n=3). ^*Significantly different from control (p<0.05). (B) Apoptosis assay using flow cytometry. PC-3 cells were treated with auranofin at various concentrations (0, 0.25, 0.5, or 1 μM) for 24 h. The cells were stained with Muse^TM annexin V dead cell kit. After incubation for 20 min at room temperature, Muse^TM cell analyzer assessed the percentage of apoptotic cells.