Fig. 5.

Knockdown of annexin A5 prevents auranofin-mediated apoptosis in PC-3 cells. (A) Cell viability assay. PC-3 cells were transfected with annexin A5 siRNA for 24 h and then treated with auranofin (1 μM) for 24 h. After incubation, the absorbance was measured at 450 nm. The percentage of cells surviving in each group relative to the control was calculated. The data are showed as a mean ± SD (n=3). ^#Significantly different from auranofin-treated cells (p<0.05). (B) Apoptosis assay using flow cytometry. PC-3 cells were transfected with annexin A5 siRNA for 24 h and then treated with auranofin (1 μM) for 24 h. The cells were stained with Muse^TM annexin V dead cell kit. After incubation for 20 min at room temperature, Muse^TM cell analyzer assessed the percentage of apoptotic cells.