Skip to main content
. Author manuscript; available in PMC: 2017 Jun 27.
Published in final edited form as: Int J Obes (Lond). 2016 Dec 27;41(3):355–359. doi: 10.1038/ijo.2016.238

Figure 2. Commonly used recombinant leptin injection concentrations are supraphysiological.

Figure 2

Plasma leptin concentrations were measured in the same three groups of wild type (WT) mice at fasting, refeeding (5 hours), and following recombinant mouse leptin injection into fasted animals. 1HL, 4HL, and 6HL refer to three separate groups of mice in which circulating leptin concentrations were measured 1, 4, or 6 hours following leptin injection. One hour following injection of recombinant mouse leptin to fasted mice (dose = 3 mg/kg body weight) plasma leptin concentrations were 170-fold higher than those at fasting and 13-fold higher than those at refeeding. By six hours following leptin injection, plasma leptin concentrations were not different from those at refeeding (n=5 WT, 10 week old male mice/group, error bars are SEM).