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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: J Immunol. 2017 Feb 3;198(6):2426–2433. doi: 10.4049/jimmunol.1601048

Figure 3. NLRP12 regulates pulmonary immune repopulation and bone marrow cell numbers after combined injury.

Figure 3

Wildtype C57BL/6 or Nlrp12−/− mice were subjected to sham or combined radiation and burn injury (RCI). Lungs were harvested 14 days post injury and the total number of (A) CD45+ cells, (B) neutrophils (CD11b+ Ly6Cint Ly6G+ F4/80−) and immature monocytes (iMOs; CD11b+ Ly6C+ Ly6GhiF4/80hi) were quantified by flow cytometry analysis. Wildtype C57BL/6 or Nlrp12−/− mice were subjected to sham or combined radiation and burn injury (RCI). Bone marrow from femurs and tibias and blood from a cheek bleed were harvested 3, 7 and 14 days post injury and the total number of (C) bone marrow cells, immature monocytes (iMOs; CD11b+ Ly6C+ Ly6GhiF4/80hi), neutrophils (CD11b+ Ly6Cint Ly6G+ F4/80−) from the bone marrow and platelets (CD62P+TER119−), monocyte and neutrophils from blood were quantified by flow cytometry analysis. Data represented as mean ± SEM, with statistical significance defined *, p<0.05, **, p<0.005 and ***, p<0.001 by Student’s t test with n=6 mice per group, with experiments performed in triplicate.