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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: J Immunol. 2017 Feb 3;198(6):2426–2433. doi: 10.4049/jimmunol.1601048

Figure 6. Anti-TNF antibody administration prevents NLRP12-associated GMP apoptosis after combined injury.

Figure 6

Wild type and Nlrp12−/− C57/BL6 mice received a single administration of anti-TNF or isotype control antibody immediately following combined radiation and burn injury (RCI). We harvested bone marrow and lung from these mice 14 days after injury. We quantified (A) the total number of bone marrow Granulocyte/Monocyte Progenitors (GMP, Lin− IL7R− Sca1− ckit+ FcγRhi CD34+) and (B) the percentage of GMP cells undergoing apoptosis by 7-AAD− Annexin V+ staining by flow cytometry. We measured (C) the total number of pulmonary CD45+ cells and immature monocytes (iMOs; CD11b+ Ly6C+ Ly6GhiF4/80hi) by flow cytometry analysis. Data represented as mean ± SEM, with statistical significance defined as *, p<0.05 by Student’s t test with n=5 mice per group, with experiments performed in triplicate.