Figure 6.

GSTA4 regulates AKT pathway in HCC cells. A. Western blot shown that the phosphorylation of AKT was elevated in MHCC97 cells transfected with GSTA4. B. The phosphorylation of AKT was perturbed in cells transfected with GSTA4 siRNA plasmid. C. Expression of D2AKT was confirmed by western blot with antibody against Flag-tag and AKT, and GAPDH was used as loading control. D. Expression of D2AKT was confirmed by Immunofluorescence assay with antibody against AKT. E. Wound healing assay was performed to determine the motility of cells co-transfected with GSTA4 silencing plasmid and D2AKT plasmid. Columns were data collected from three independent experiments. Representative pictures were taken after staining with crystal violet. Data were from three independent experiments and are average ± SD. **P < 0.01, compared to control siRNA cells; ##P < 0.01, compared to GSTA4 siRNA cells. F. Representative pictures of (left panel) and quantification (right panel) of invasion in MHCC97 cells transfected with GSTA4 siRNA or D2AKT plasmid were analyzed using a Transwell invasion assay. Data were from three independent experiments and are average ± SD. **P < 0.01, compared to control siRNA cells; ##P < 0.01, compared to GSTA4 siRNA cells.