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. 2017 Feb 15;9(2):301–315.

Figure 7.

Figure 7

GSTA4 regulates HCC cells metastasis through AKT pathway. A. In the presence of GSK690693, HepG2 cells were incubated for 1 h, protein extracts were analyzed by western blot with antibodies against phosphorylated AKT (S473) or AKT. B. Expression of AKT was confirmed by Immunofluorescence assay with antibody against AKT. C. In the presence of GSK690693, wound healing migration assay was conducted to evaluate the cell motility after transfection. Data were from three independent experiments and were average ± SD. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected GSTA4 cells. D. In the presence of GSK690693, Transwell assay was conducted to evaluate the cell invasion after transfection. Data were from three independent experiments and were average ± SD. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected GSTA4 cells.