Figure 5.

Long term low- and high-dose As affected cell proliferation and growth. Cell proliferation and growth of T24 cells, following As exposure (0, 200 or 1,000 nM) for 100 days, were assayed. A. The number of EDU positive cells (red) or of the nuclei (blue) and the ratio of EDU positive cells/all cells were labeled on the up-right, respectively. Cell proliferation increased following As exposure at 200 nM, but decreased following exposure at 1,000 nM. B. Cell growth was examined using WST assay. Cell growth increased following As exposure at 200 nM, but decreased following exposure at 1,000 nM. (*P<0.05).