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. 2017 Feb 15;9(2):507–519.

Figure 3.

Figure 3

Knockdown of AGR2 represses cell proliferation of AdCC cell lines in vitro and in vivo. A. Cell viability assays on SACC-83 and SACC-LM cells. Knockdown of AGR2 significantly repressed the proliferation of SACC-83 and SACC-LM cells on Day4 and 5 (Mean ± SEM, **P<0.01, ***P<0.001). B. Anchorage-dependent colony formation assay of SACC-83 and SACC-LM cells. Knockdown of AGR2 significantly attenuated the colony formation ability of both SACC-83 cells and SACC-LM cells (Mean ± SEM, **P<0.01, unpaired t test). C. Representative images of the tumors of negative control group (NC, n = 5) and AGR2 shRNA group (shAGR2, n = 5). D. Tumor size of NC group and shAGR2 group was assessed (Mean ± SEM, **P<0.01, ***P<0.001 unpaired t test). E. Western blotting indicated that knockdown of AGR2 reduced the protein level of Survivin and Cyclin D1 in SACC-83 cells and SACC-LM cells. The β-actin was used as loading control. F. Knockdown of AGR2 significantly reduced the mRNA levels of Survivin and Cyclin D1 in SACC-83 cells and SACC-LM cells (Mean ± SEM, **P<0.01, ***P<0.001, unpaired t test).