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. 2017 Feb 15;9(2):507–519.

Figure 5.

Figure 5

Knockdown of AGR2 reverses the EMT phenomenon induced by TGF-β1. A. Representative Immunofluorescence photos of SACC-LM treated with negative control shRNA (NC), TGF-β1 with or without AGR2 shRNA (shAGR2) were displayed. The expression of E-cadherin was reduced by TGF-β1 but reversed by AGR2 knockdown. The expression of N-cadherin was up-regulated by TGF-β1 but recovered by AGR2 knockdown (scale bar = 10 μm). B. Western blotting showed the expression of E-cadherin, N-cadherin, Slug and CD147 in SACC-83 and SACC-LM cells tranfected with negative control shRNA (NC) or with AGR2 shRNA (shAGR2) in the presence of TGF-β1. The β-actin was used as loading control. C. The mRNA levels of Slug, Snail and CD147 in SACC-83 and SACC-LM cells were significantly reduced by AGR2 silencing in the presence of TGF-β1 (Mean ± SEM, **P<0.01, ***P<0.001, unpaired t test). D. Knockdown of AGR2 reversed the enhanced ability of migration which induced by TGF-β1 in SACC-83 cells and SACC-LM cells (Mean ± SEM, **P<0.01, ***P<0.001, one way ANOVA post tukey test, scale bar = 100 μm).