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. 2017 Feb 15;9(2):520–532.

Figure 2.

Figure 2

CMyb is a target gene of miR-15a in HCC cells. A: Schematic illustration of the conserved miR-15a binding sites. The cMyb 3’-UTR contains two predicted miR-15a binding sites. The seed regions of miR-15a and the seed-recognizing sites in the cMyb 3’-UTR are indicated in white background, and all nucleotides in seed-recognizing sites are completely conserved across several species. B: Western blotting analysis of cMyb protein levels in MHCC97H and Huh7 cells transfected with miR-15a mimic or inhibitor. C: Direct recognition of the cMyb 3’-UTR by miR-15a. Firefly luciferase reporters individually containing wild-type (WT), mutant (MUT)1, MUT2 or MUT1,2 miR-15a binding sites in the cMyb 3’-UTR were co-transfected into HEK293T cells with the scrambled negative control RNA, miR-15a mimic or inhibitor. At 24 h post-transfection, the cells were treated with a luciferase assay kit. The results were calculated as the ratio of firefly luciferase activity in the miR-15a-transfected cells normalized to the control cells. *P < 0.05; **P < 0.01.