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. 2017 Feb 15;9(2):520–532.

Figure 3.

Figure 3

CMyb expression is increased in HCC sphere culturing cells and is positive correlated with the mobility of HCC cells. A: Western blotting analysis and quantification of cMyb protein levels in MHCC97H and Huh7 sphere culturing cells and their relative parental cells. B: Western blotting analysis and quantification of cMyb protein levels in MHCC97H cells transfected with control siRNA or cMyb siRNA. Western blotting analysis and quantification of cMyb protein levels in Huh7 cells transfected with control plasmid or cMyb overexpression plasmid. C: Left panel: Representative image of transwell assay of MHCC97H cells transfected with control siRNA or cMyb siRNA. Right panel: Quantitative analysis of migration rates. C: Left panel: Representative image of Transwell assay of Huh7 cells transfected with control plasmid or cMyb overexpression plasmid. Right panel: Quantitative analysis of the migration rates. *P < 0.05; **P < 0.01 (MHCC97H parental cells = MHCC97H/p, MHCC97H sphere culturing cells = MHCC97H/s, Huh7 parental cells = Huh7/p, Huh7 sphere culturing cells = Huh7/s).