Skip to main content
. 2017 Feb 15;9(2):591–600.

Figure 4.

Figure 4

miR-146a directly targets TGF-β1. A and B. HUVECs were transfected with miR-146a and subjected to qRT-PCR analysis of miR-146a and TGF-β1 expression, respectively. C. Supernatants from miR-146a-transfected HUVECs were subjected to ELISA analysis of TGF-β1 protein expression. D. The predicted miR-146a target sequence in the 3’-UTR of TGF-β1 and the mutantcontaining eight mutated nucleotides (TGF-β1-mut). E. HUVEC cells were transfected with a reporter vector containing the WT (wild-type) or MUT (mutant) TGF-β 3’-UTR along with a control mimic (control) or miR-146a mimics (miR-146a). HUVECs were co-transfected with miR-146a and luciferase reporters containing either the predicted miRNA target site in the TGF-β1 3’-UTR or its corresponding mutant 48 hours after transfection. Levels were normalized to Renilla luciferase activity. F and G. TGF-β1 mRNA and protein levels were assessed by qRT-PCR and ELISA, respectively, in LPS-induced HUVECs.