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. 2017 Feb 15;9(2):629–637.

Figure 4.

Figure 4

HSP90 mRNA is a direct target of hsa-miR-148b in VSMCs. A. The predicted hsa-miR-148b binding site in the HSP90 3’-UTR is shown. B. The expression of hsa-miR-148b was significantly negatively correlated with HSP90 mRNA levels in plaques of atherosclerotic patients. C. qRT-PCR and western blot analysis of HSP90 expression in VSMCs transfected with the hsa-miR-148b mimic or mimic control. D. Hsa-miR-148b inhibited luciferase activity in the HSP90 with WT binding site 3’-UTR, but luciferase activity was not decreased in the MUT binding site 3’-UTR of HSP90 mRNA. WT: wild type; MUT: mutant. LUC: luciferase. **P<0.01.