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Hypoxic preconditioning stabilizes HIF-1α in cell sheets. CDC monolayer sheets were incubated under normoxia or hypoxia for 24 hours. HIF-1α expression was evaluated using immunocytochemistry (A) and western blotting (B). DMOG, a PHD inhibitor, was used as a positive control for HIF-1α expression in immunocytochemistry. Normo: Normoxia; Hypo: Hypoxia. Scale bar = 50 μm.
