Figure 2.

Induced Splicing and expression of XBP1 by ER stress is critical for growth of cancer cells in 3D culture. (A) RT-PCR showed Tm/Tg promotes splicing of XBP1. (B) higher expression of XBP1s after Tm/Tg treatment. (C) Western blot showed the expression level of spliced XBP1 (XBP1s). (D) Overexpression of XBP1s promotes number of spheroids in 3D culture. (E) Quantitative analysis of (D). ***P<0.001. (F) Knockdown of XBP1s by siRNA decreases number of spheroids in 3D culture. (G) Quantitative analysis of (F). ***P<0.001. (H) The size of spheroids showed no significance between control cells and XBP1s overexpressing cells. Data are expressed as the mean numbers of independent triplicate experiments.