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. 2017 Feb 15;9(2):700–707.

Figure 4.

Figure 4

XBP1 promotes A549 growth in 3D culture by regulating expression of LOX. A: Prediction of binding site of XBP1 in the promoter of LOX. We used the DECODE transcription factor database to predict transcription factors that may bind to the promoter region of LOX. The result showed XBP1 may bind at the 5’UTR of mRNA of LOX. B: QPCR showed overexpression of XBP1s upregulated the expression of LOX in both 2D and 3D culture at mRNA level. ***P<0.001. C: Westernblot showed higher expression of LOX in XBP1s overexpressing cells. D: QPCR showed knockdown of XBP1s downregulated the expression of LOX in both 2D and 3D culture at mRNA level. ***P<0.001. E: Westernblot showed decreased expression of LOX after knockdown of XBP1s. F: Luciferase reporter assay showed direct binding of XBP1 on the promoter of LOX mRNA. ***P<0.001. Data are expressed as the mean numbers of independent triplicate experiments.