Skip to main content
. 2017 Feb 15;9(2):715–721.

Figure 4.

Figure 4

MiR-21 specifically inhibits LRP6 expression. A. Putative binding site of miR-21 in the 3’-UTR of LRP6 mRNA and the mutated nucleotide residues are shown in red and italics, respectively. B. HepG2 cells were co-transfected with 0.2 µg LRP6 3’-UTR-Luc or LRP6 3’-UTR-mut-Luc and 25 nM miR-21 mimic or control. C. HepG2 cells were transiently co-transfected with LXRa 3’-UTR-Luc and 25 nM of the indicated RNA oligonucleotides. Luciferase assays were conducted 48 h post-transfection. Data represent the firefly luciferase activities normalized with respect to the β-galactosidase activities. **P < 0.01 represents the relative luciferase activity of the LRP6 3’-UTR-mut group compared with the LRP6 3’-UTR group.