Table 1.
Human primer sequences for qRT-PCR analysis
| Target Gene | Reverse | Forward | Accession |
|---|---|---|---|
| LRP6 | Forward | 5’-CAGTTGGAGTGGTGCTGAAAGG-3’ | NM_002336 |
| Reverse | 5’-CCATCCAAAGCAGCCCGTTCAA-3’ | ||
| ACC1 | Forward | 5’-TTCACTCCACCTTGTCAGCGGA-3’ | NM_000664 |
| Reverse | 5’-GTCAGAGAAGCAGCCCATCACT-3’ | ||
| FASN | Forward | 5’-TTCTACGGCTCCACGCTCTTCC-3’ | NM_004104 |
| Reverse | 5’-GAAGAGTCTTCGTCAGCCAGGA-3’ | ||
| SCD1 | Forward | 5’-CCTGGTTTCACTTGGAGCTGTG-3’ | NM_005063 |
| Reverse | 5’-TGTGGTGAAGTTGATGTGCCAGC-3’ | ||
| LXRα | Forward | 5’-TGGACACCTACATGCGTCGCAA-3’ | NM_001130101 |
| Reverse | 5’-CAAGGATGTGGCATGAGCCTGT-3’ | ||
| SREBP1 | Forward | 5’-ACTTCTGGAGGCATCGCAAGCA-3’ | NM_001005291 |
| Reverse | 5’-AGGTTCCAGAGGAGGCTACAAG-3’ |
ACC1, acetyl-CoA carboxylase 1; FASN, fatty acid synthase; SCD1, stearoyl CoA desaturase 1; LXRa, liver X receptor; SREBP1, sterol regulatory element-binding protein 1; LRP6, low-density lipoprotein related receptor 6.