Figure 4.

IL6 and TNF induce NR4A1 in chondrocytes. The cultured chondrocytes from normal joint cartilage are treated with IL6 or TNF for 12 or 24 h, after which western blot is performed to examine NR4A1 expression. GAPDH is an internal reference. The experiments are repeated five times. Both IL6 and TNF induce NR4A1 protein expression in a time-dependent manner based on results from the two time points. NR4A1, nuclear receptor subfamily 4, group A, member 1. IL6, interleukin 6. TNF, tumor necrosis factor.