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Images from delayed microscopy of neurites of rat hippocampal neurons after treatment with LPA and Y27632 (200×). In the control group, the number and length of protrusions increased dramatically with the time. In LPA group, LPA was added to activate Rho kinase. Protrusions rapidly shortened with the time. In Y27632+LPA group, the first, second, third and fourth-level protrusions continued to extend and grow, and neurons showed favorable polarity.
