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. 2017 Mar 8;8:113. doi: 10.3389/fphar.2017.00113

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Copyright © 2017 Zhang, Zhang, Wang and Si.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ginsenoside Rg3 reduces PPAR-γ mRNA expression in 3T3-L1 cells. On day 10, cells treated with various concentrations of Rg3 were collected to measure PPAR-γ mRNA expression by quantitative real time PCR and normalized by β-actin expression. The same membrane was stripped using a stripping buffer and blocked and probed with a new primary antibody to detect the next specific protein. Changes by treatment were expressed as the percentage of DMSO control. Data are expressed as mean ± SE, n = 3. ^∗P < 0.05, ^∗∗P < 0.01 vs. DMSO control.