FIGURE 4.

Ginsenoside Rg3 dose-dependently attenuates protein expressions of fat packing proteins FAS (A) and perilipin (B) in 3T3-L1 cells. On day 10, cells treated with various concentrations of Rg3 were collected to measure FAS and perilipin protein expressions by western blotting and normalized by β-actin expression. The same membrane was stripped using a stripping buffer and blocked and probed with a new primary antibody to detect the next specific protein. Changes by treatments were expressed as the percentage of DMSO control. Data are expressed as mean ± SE, n = 3. A set of representative images and bar graph were shown. ^∗P < 0.05, ^∗∗P < 0.01 vs. DMSO control.