Figure 6.

Bone marrow-derived cells accumulate more rapidly and to a greater extent in mutant SOD1 (mSOD) mice conditioned with a high dose of BU compared to a lower dose. (A) Weekly quantification of GFP⁺ cells within the lumbar spinal cord in mSOD mice conditioned with either 80 or 125 mg/kg BU and transplanted at 15 weeks of age. Data = mean ± SEM for n = 3 mice/timepoint. (B) Weekly quantification of GFP⁺ cell morphology within the lumbar spinal cord in mSOD mice conditioned with 80 mg/kg BU and transplanted at 15 weeks of age. Data = mean ± SEM for n = 3 mice/timepoint. (C) Weekly quantification of GFP⁺ cell morphology within the lumbar spinal cord in mSOD mice conditioned with 125 mg/kg BU and transplanted at 15 weeks of age. Data = mean ± SEM for n = 3 mice/timepoint. (D) Weekly quantification of Ki67⁺ cells and GFP⁺Ki67⁺ cells within the lumbar spinal cord in mSOD mice conditioned with either 80 or 125 mg/kg BU and transplanted at 15 weeks of age. Data = mean ± SEM for n = 3 mice/timepoint. (E) Weekly quantification of Iba1⁺ cells within the lumbar spinal cord in mSOD mice conditioned with either 80 or 125 mg/kg BU and transplanted at 15 weeks of age. Data = mean ± SEM for n = 3 mice/timepoint. (F) Immunohistochemical analysis of lumbar spinal cord sections from late stage mSOD mice that were conditioned with 125 mg/kg BU and transplanted with GFP⁺ donor BM cells at 15 weeks of age. Mice were given IP injections of 50 mg/kg 5-ethynyl-2′-deoxyuridine (EdU) 48 and 24 h prior to collection to label proliferating cells. Nuclei are shown in blue, EdU in red, and GFP⁺ cells in green. Results are representative images from n = 3 mice. Data were analyzed by ANOVA, and post hoc tests using the Tukey–Kramer method were used. * represents a significant difference between 80 and 125 mg/kg treatment groups with p < 0.01; ŧ represents a significant difference from the preceding timepoint with p < 0.01; Ŧ represents a significant difference from the preceding timepoint with p = 0.02.