Figure 2.

Dendritic cells (DCs) migrate toward supernatants (SN) of CT26 cells and are particularly activated by SN of irradiated CT26 cells. Bone marrow-derived DCs from BALB/c mice (mDCs) were harvested and seeded to the upper chamber of a transmigration system (3.0 μm pore size). The lower chamber was filled with cell culture SN obtained from CT26 tumor cells 24 h after irradiation with 2 × 5 Gy on consecutive days or with SN of mock-treated cells. After 14 h of incubation at 37°C, the transmigration index (MI), reflecting the migration of mDCs toward SN of the tumor cells versus the medium only control, was determined (A) and the expression of CD80 (B) and CD86 (C) on the MHCII⁺ transmigrated cells was determined by flow cytometry. Joint data of three independent experiments are presented as mean ± SEM and analyzed by Student’s t-test; **p < 0.01.