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. 2017 Mar 8;8:231. doi: 10.3389/fimmu.2017.00231

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Copyright © 2017 Frey, Rückert, Weber, Mayr, Derer, Lotter, Bert, Rödel, Fietkau and Gaipl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The activation of dendritic cells (DCs) by supernatants (SN) of irradiated CT26 cells is independent of the migration. Bone marrow-derived DCs from BALB/c mice (mDCs) were incubated at 37°C in SN obtained from CT26 tumor cells 24 h after irradiation with 2 × 5 Gy on consecutive days, in SN of non-irradiated mock treated CT26 cells, or in medium containing lipopolysaccharide (LPS). The expression of CD80 (A) and CD86 (B) on mDCs was analyzed after 24 and 48 h via flow cytometry. Representative data of one out of three independent experiments each performed in triplicates are presented as mean ± SEM and analyzed by Student’s t-test; **p < 0.01.