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. 2017 Mar 8;8:265. doi: 10.3389/fimmu.2017.00265

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Copyright © 2017 Gonzalez-Perez and Lamousé-Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine production of control (CTRL) and maternal antibiotic treatment (MAT) TLR4KO and MyD88KO effector CD8⁺ T cells in response to lipopolysaccharide (LPS). Pooled CD8⁺ T cells isolated from the spleens of uninfected day of life 15 CTRL TLR4KO (n = 2), MAT TLR4KO (n = 5), CTRL MyD88KO (n = 5), and MAT MyD88KO (n = 4) infant mice were stimulated with anti-CD3 and anti-CD28 with or without Escherichia coli-derived LPS (1 μg/ml) for 72 h. Analysis of the percentage of interferon gamma (IFN-γ) and TNF-α-producing effector CD8⁺ T cells (CD44⁺CD62L⁻) was performed by flow cytometry. DN, double negative for IFN-γ and TNF-α expression. Data are representative of two independent experiments.