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. 2017 Apr;23(4):567–577. doi: 10.1261/rna.060236.116

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© 2017 Friesen et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Identification of premature stop codon readthrough activity of clitocine. (A) Schematic of luciferase reporter constructs used in high-throughput screens and assays to identify and characterize compounds with readthrough activity. (B) Structure of clitocine (2R,3R,4S,5R)-2-([6-amino-5-nitropyrimidin-4-yl]amino)-5-(hydroxymethyl) tetrahydrofuran-3,4-diol. Premature stop codon readthrough activity of clitocine in the UAA (C), UGA (D), UAG (E), and wild-type (F) luciferase reporter assays compared to that of the aminoglycosides, gentamicin, and G418. Each point is the average of three determinations and error bars represent the standard deviation of the mean.