FIGURE 3.

Clitocine is incorporated into RNA in cells. CALU-6 cells were labeled with ortho [³²P]phosphate and incubated with 1 µM clitocine (B and D) or without (A and C). After 24 h, total RNA (C and D) and poly(A) RNA (A and B) was isolated and digested with nuclease P1. Nucleotides were purified, separated by 2D TLC, and visualized by Phosphorimager analysis. The arrow in panels B and D indicates clitocine-3′-phosphate. The separation pattern of the 3′-phosphate nucleotides is the same as in Figure 3.