FIGURE 2.

Compensatory mutation experiment to establish the register between the C terminus of PPR10 and the 3′-region of the atpH binding site. (A) Diagram of PPR10 aligned in two proposed registers to the atpH binding site. Register 1 was proposed in Barkan et al. (2012) and Register 2 was proposed in Yin et al. (2013). To distinguish between the registers, the nucleotide specifying amino acids in Repeat 16 were changed from 6N,1′D to 6T,1′N, which is predicted to change the bound nucleotide from U to A. (B) Gel mobility shift assays showing that PPR10 variant Rpt16(TN) binds preferentially to the U14A substituted atpH binding site, supporting Register 2. This assay was repeated three times with similar results. A representative experiment is shown.