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. 2004 Nov 8;5:86. doi: 10.1186/1471-2164-5-86

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Copyright © 2004 Milona et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time course of effect of 100 ng/mL BMP-2 treatment on C3H10T1/2 cells. Murine Sp8 and beta-actin mRNA expression was determined by RT real time PCR. The relative levels of expression in treated (•) and untreated cells (◆) were examined after 0, 7, 14 and 20 days treatment. Significant increases in Sp8 expression were seen at 7, 14 and 20 days (p < 0.05).