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. 2017 Mar;27(3):491–499. doi: 10.1101/gr.209601.116

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© 2017 Smith et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 3. — UMI-tools improves reproducibility between iCLIP replicates. (A) Average edit distances between UMIs with the same alignment coordinates. Genomic positions with a single UMI are not shown. (Null) Null expectation from random sampling of UMIs, taking into account the genome-wide distribution of UMIs. Only the first replicate of the data set is shown for each pull down. (B) iCLIP reproducibility as represented by the percentage of positions with more than two tags also cross-linked in at least one of two other replicates. (C) Spearman's rank correlation between the numbers of significant tags in each exon.