Table 1.
List of primers utilized in quantification of gene expression by semi-quantitative RT-PCR.
| Gene | Protein | Primer | Sequence |
|---|---|---|---|
| glcH | Glucose transporter | Forward | GCTTTTATGGCAGGTTCTTT |
| Reverse | CAAATAGCCGCAAGACTCAG | ||
| prk | Phosphoribulokinase | Forward | ATGGTGGTGTTGTTGGAGAT |
| Reverse | CCTTTCAGCA TTGTCACGAT | ||
| fbaA | Fructose 1-6 biphosphate aldolase | Forward | ATAGGTGAAGAGGTTGGGAG |
| Reverse | ATCAGAAGCAGCCAATACAG | ||
| glpA | Glucose-1P-adenylyltransferase | Forward | TTGCGTTTTAGGCGTTAGAA |
| Reverse | TCGTTCCCTGACCTACTCCA | ||
| tal | Transaldolase | Forward | TATTTGGCTTTTCTCAGGC |
| Reverse | GTCCTTCCCTGTTTCTGCT | ||
| gap3 | Glyceraldehyde-3-phosphate dehydrogenase | Forward | GGTAACGCAAGAAGAAGTCA |
| Reverse | TGAGTTTTGTTGACCACCAT | ||
| prk | Pyruvate kinase | Forward | TTCAATTGGTATGGGGTGTA |
| Reverse | GGGTTCCTGCTGTTTCTACT | ||
| rbcS | Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit | Forward | CCTGGAGACA TTTGGCTT |
| Reverse | GTCTGCA TGGAGCCACTT | ||
| rbcL | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | Forward | TCGCCCTCTTGTTGGTTG |
| Reverse | CCTGGCCTGTCTGTTGCT | ||
| rnpB | RNase subunit B | Forward | TCCCAAAAGACCAGACTTGCT |
| Reverse | GTATGTTTCTGTGGCACTCTCCT | ||
| rnpB | RNase subunit B (used in natural samples; Holtzendorff et al., 2002) | Forward | GGCAAGGGTGCAAAGGTG |
| Reverse | GCCGGGGTTTACCTAGCC |