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Variability Sources in ANOVA Model (See Figure 1). Mixed ANOVA Model:Xij = u + ai + Bj + Eij where Xij is the observation (LN intensity), ai is the tissue storage effect, Bj is the individual variability effect and Eij is the noise term.
| Variability Source | Type | df |
| Tissue Storage | Fixed | 3 |
| Woman, Individual Variation | Random | 2 |
| Interaction (Woman × Storage) | Random | 6 |
| Replication (RNA isolation, chip Processing) | Random | 12 |
