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ROP18 promotes degradation of UBE2N. A, 293T cells were transfected with V5-ROP18Δ83 or V5-ROP18Δ83-KD plasmids for 36 h, and then total cell lysates were subjected to Western blotting analysis. IB, immunoblot. B, 293T cells were infected without or with wild-type (WT) RH or ROP18-Ty1 RH strain for 36 h. Cell lysates were separated by SDS-PAGE and immunoblotted with the indicated antibodies. SAG1 and GAPDH were used as loading controls.
