Figure 3. Sandwich culture: Relative abundance of mRNA expression of apoptosis markers (BCL2L, BAX and FAS) as well as GHR1A and IGF-1 from day 0 to day 14 (cells after Easycoll isolation). The urea and LDH concentration was determined in the cell culture medium supernatant but not after purification of cells (day 0). Significant differences were indicated as follows: * P < 0.05, ** P < 0.01, *** P < 0.001. BCL2L = B-cell lymphoma 2 like 1, BAX = BCl2 associated X, FAS = Fas (CD95L), LDH = Lactate dehydrogenase, GHR1A = Growth hormone receptor 1A transcript, IGF-1 = Insulin-like Growth Factor-1.